Vascular Smooth Muscle and Cardiac Cells
نویسندگان
چکیده
The presence of chloride-formate anion exchange in vascular smooth muscle cells (VSMCs) and cardiac myocytes was investigated. Imposing an outward chloride gradient in sarcolemmal microsomes isolated from canine aorta stimulated [W4C]formate uptake compared with the absence of a chloride gradient (24.3±2.33 versus 9.8±1.41 pmol/mg protein for 30 seconds, P<.03) and induced transient uphill ['4C]formate uptake. The chloride-formate exchange was significantly inhibited in the presence of 1 mmol/L 4,4'-diisothiocyanostilbene2,2'-disulfonic acid (DIDS) or furosemide (57% and 61%, respectively). Incubation of rat cultured VSMCs in a medium containing [14C]formate resulted in uptake of formate that was significantly DIDS and furosemide sensitive (79.34-2.47, 43.03 ±2.37, and 44.65+1.68 pmol/mg protein for 4 minutes in control, DIDS, and furosemide groups, respectively). Preincubation of the VSMCs in chloride-free medium significantly reduced the DIDS-sensitive (36.31 versus 16.85 pmol/mg
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